Detailed material

Evidence-Based Complementary and Alternative Medicine

Publication Date: year 2011

Title of Article：

Targeting PML-RARα and Oncogenic Signaling Pathways with Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells.

Research author：

Lai Gi-Ming, Yao Chih-Jung, Shuang-En Chuang,
Jiann-Long Yan, Kun-Huang Yan, Tung-Yuan Lai,
Chia-Ming Yang, Chun-yen Liu, Suz-Wen Chen

Research unit：

Cancer Research Center ,Wan Fang Hospital, Taipei Taiwan
Formosa Cancer Foundation, Taiwan
National Health Research Institutes, Taiwan
College of Life Science, National Tsing Hua University, Taiwan
School of Chinese Medicine, China Medical University, Taiwan
Chinese Internal Medicine, China Medical University Hodpital, Taiwan

Research unit Conclusions：

In this study, we explored the effects of THL on acute promyelocytic leukemia (APL) NB4 cells, which could be effectively treated by some traditional Chinese remedies containing arsenic trioxide. The results showed THL could induce G2/M arrest and apoptosis in NB4 cells. Accordingly, the decrease of cyclin A and B1 were observed in THL-treated cells. The THL-induced apoptosis was accompanied with caspase-3 activation and decrease of PML-RARα fusion protein. Moreover, DNA methyltransferase 1 and oncogenic signaling pathways such as Akt/mTOR, Stat3 and ERK were also down-regulated by THL. By using ethyl acetate extraction and silica gel chromatography, an active fraction of THL named as EAS5 was isolated. At about 0.5–1% of the dose of THL, EAS5 appeared to have most of THL-induced multiple molecular targeting effects in NB4 cells. Based on the findings of these multi-targeting effects, THL might be regarding as a complementary and alternative therapeutic agent for refractory APL.

Journal Introduction：

The Evidence-Based Complementary and Alternative Medicine was established in 2004 by Professor Edwin L. Cooper. It is a medical journal with an international prospective that mainly collects papers on complementary and alternative medicine and encourages exploring folk medicine and new therapies for further understanding and examination.

Impact Factor for 2011 is 4.774.

Link to Journal：

website address：http://www.hindawi.com

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